Application

Monoclonal Anti-MASTL antibody produced in mouse may be used in various immunochemical techniques including immunoblotting, flow cytometry and immunofluorescence.

Biochem/physiol Actions

Microtubule-associated serine/Threonine kinase-Like (MASTL) acts as a cell cycle marker of M phase. It has an ability to regulate mitosis entry, anaphase and cytokinesis. In Drosophila MASTL (Greatwall) was initially identified as a kinase essential for chromosome condensation. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events. Furthermore, MASTL is also an inhibitor of the tumor suppressor protein Phosphatase 2A (PP2A), and thus maintains the equilibrium between cyclin B-Cdk1 and PP2A. A missense mutation in the MASTL gene leads to the development of autosomal dominant inherited thrombocytopenia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-MASTL (mouse IgG2b isotype) is derived from the hybridoma 2C8/D11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein. Microtubule-Associated Serine/Threonine kinase-Like (MASTL), also known as GREATWALL (GWL) in Drosophila and Xenopus is localized to the nucleus but, during M-phase it is activated and re-localized to the centrosomes.

Immunogen

immunized with a fusion protein of 250aa polypeptide from the region that divides the kinase domain of mouse MASTL

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.